Abscess |
- Aspiration of exudate from intact abscess
- Exudate
- Section of abscess wall
- Swab
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- Red top tube
- Bacterial transport system
- Sterile bag or jar
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Blood |
- Surgical prep
- 2-3 samples ideal
- Sample when febrile if possible
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CSF |
- Surgical prep
- Larger volume preferable due to bacterial numbers
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- Blood culture bottle
- Fluid transport vial
- Sterile container/RTT
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- Blood culture OR Aerobic, Anaerobic
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Dermatophyte |
- Clean lesion with 70% alcohol
- Pluck hair from lesion edge
- Toothbrush
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Eye |
- Remove crusts prior to sampling
- Topical dyes and anesthetics may interfere — rinse first
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- Bacterial transport system
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Feces |
- ~5 g per rectum or immediately after defecation
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- Sealed, leak-proof jar or bag
- Cary-Blair for Salmonella only
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Joint |
- Surgical prep and collect fluid
- Synovium
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- Blood culture bottle
- Fluid transport vial
- Sterile container
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- Blood culture OR Aerobic, Anaerobic, Optional: Mycoplasma
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Lower Respiratory |
- Transtracheal Wash
- Bronchoalveolar Lavage
- Aspirate
- Avoid upper respiratory tract sampling when possible
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- Submit entire TTW or BAL volume in a sterile container
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- Aerobic, Optional: Mycoplasma
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Milk |
- 3-5 ml after teat disinfection and stripping
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- Refrigerate or freeze if ³48 hour delay
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- Aerobic, Optional: Mycoplasma
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Outer Ear |
- Clean debris from canal with saline
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- Bacterial transport system (gel systems may interfere with Gram stains)
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Upper Respiratory |
- Biopsy
- Swab of nasal cavity or nasopharynx
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- Sterile container
- Bacterial transport system
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Urine |
- Cystocentesis
- Clean catheterization
- Mid-stream free catch
- Indwelling catheter samples are suboptimal
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Uterine |
- Double guarded swab
- Biopsy
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- Transport system
- Sterile container
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Skin |
- Surgical prep and biopsy
- Pustule aspiration
- Swab of affected area after cleaning
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- Sterile container
- Transport system
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Necropsy |
- Use sterile instruments
- Collect samples before opening the GI tract
- Tissue samples are ideal – should be ~5g
- Place each tissue in a separate bag
- Tie off the ends of segment of intestine
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- Whirl-Pak Bags
- Sterile Container
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